GPF Symposium 2023

Contribution List

1. Oliver Valerius – Georg-August-Universität Göttingen

29/06/2023, 10:00

Session 1 : Proximity labeling and mass spectrometry of native protein complexes

Analysis of protein proximities with biotin labeling and mass spectrometry (BioID) – LCMS core facility support for BioID

Together with the Göttingen Proteomics Forum (GPF) our university proteomics core facility applied for and was granted DFG funding to establish support and service capacity for research groups that intend to start and conduct (split-)BioID experiments in cooperation...

2. Julien Béthune – Hochschule für Angewandte Wissenschaften Hamburg

29/06/2023, 10:20

Session 1 : Proximity labeling and mass spectrometry of native protein complexes

UltraID, a small-size hyperactive enzyme for proximity-dependent biotinylation

Proximity-dependent biotinylation (PDB) has become a standard technique in the toolbox of methods available for the identification of protein-protein interactions. The most commonly used approach is based on the original BioID technique in which an abortive variant of a prokaryotic biotin protein ligase is fused...

3. Nina Morgner – Goethe-Universität Frankfurt

29/06/2023, 11:00

Session 1 : Proximity labeling and mass spectrometry of native protein complexes

Native mass spectrometry: How to probe molecular principles of assembly and interactions of protein complexes

Nina Morgner, Rene Zangl, Jonathan Schulte

Protein complex assembly as well as their interplay are controlled by the non-covalent interactions of all biomolecular partners. Native mass spectrometry and ion mobility are ideally suited to unravel the molecular principles which...

4. Henning Urlaub – MPI für Multidisziplinäre Naturwissenschaften, Göttingen

29/06/2023, 12:40

Session 2 : Crosslinking

RNA/DNA-protein crosslinking mass spectrometry - Applications of UV and chemical crosslinking

Crosslinking mass spectrometry (XL-MS) can resolve sites of interaction in protein-RNA and DNA complexes at single amino acid and nucleic acid resolution. Processing of (large) XL-MS data, reliable assignment, and visualization of crosslinking results – including annotation of spectra –remain key...

5. Andrew Norris – Thermo Fisher Scientific

29/06/2023, 13:20

Session 2 : Crosslinking

Bound Together: The picture-perfect marriage of crosslinking mass spectrometry and Cryo-EM

Yuqi Shi(1); Ye Hi(1); Leigh Foster(2), Albert Konijnenberg(3), Ryan Bomgarden(2), Rosa Viner(1)

Thermo Fisher Scientific, (1) San Jose, CA, (2) Rockford, IL, (3) Eindhoven, NL

Cross-linking mass spectrometry (XL-MS) has become a powerful structural tool for mapping protein-protein interactions...

6. Juri Rappsilber – Technische Universität Berlin

29/06/2023, 13:40

Session 2 : Crosslinking

Protein structures inside cells – how and why

Accurately modelling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Remaining challenges result from the limited information available on specific protein states including protein-protein interactions. We present how experimental data enable systematically modelling novel...

7. Ralf-Peter Jansen – Eberhard Karls Universität Tübingen

29/06/2023, 14:50

Session 3: Biotin Identification (BioID)

(RNA-)BioID as tool to probe for RNA-protein association

8. Noa Lipstein – Leibniz Forschungsinstitut für Molekulare Pharmakologie, Berlin

29/06/2023, 15:30

Session 3: Biotin Identification (BioID)

A Proximity Map of the Presynaptic Terminal

In the brain, information is transferred between neurons at synaptic connections. Synapses show a high degree of structural and functional diversity, and undergo continuous adaptation during development, in response to stimuli, and in disease. To discover molecular mechanisms that underlie synaptic diversity and plasticity, we established...

9. Closing remarks

29/06/2023, 16:10

Session 3: Biotin Identification (BioID)

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