GPF Symposium 2023

Session

Session 1 : Proximity labeling and mass spectrometry of native protein complexes

Jun 29, 2023, 10:00 AM

MPI-NAT City Campus

1. Oliver Valerius – Georg-August-Universität Göttingen

6/29/23, 10:00 AM

Analysis of protein proximities with biotin labeling and mass spectrometry (BioID) – LCMS core facility support for BioID

Together with the Göttingen Proteomics Forum (GPF) our university proteomics core facility applied for and was granted DFG funding to establish support and service capacity for research groups that intend to start and conduct (split-)BioID experiments in cooperation...

2. Julien Béthune – Hochschule für Angewandte Wissenschaften Hamburg

6/29/23, 10:20 AM

UltraID, a small-size hyperactive enzyme for proximity-dependent biotinylation

Proximity-dependent biotinylation (PDB) has become a standard technique in the toolbox of methods available for the identification of protein-protein interactions. The most commonly used approach is based on the original BioID technique in which an abortive variant of a prokaryotic biotin protein ligase is fused...

3. Nina Morgner – Goethe-Universität Frankfurt

6/29/23, 11:00 AM

Native mass spectrometry: How to probe molecular principles of assembly and interactions of protein complexes

Nina Morgner, Rene Zangl, Jonathan Schulte

Protein complex assembly as well as their interplay are controlled by the non-covalent interactions of all biomolecular partners. Native mass spectrometry and ion mobility are ideally suited to unravel the molecular principles which...