Conference: Protein Folding on the Ribosome

Dec 15, 2024, 3:00 PM → Dec 18, 2024, 2:00 PM Europe/Berlin

Harnack House

Prof. Marina Rodnina

Following the exciting meetings in Cambridge, Stockholm, Berlin, and Baltimore, we will continue the discussion of the emerging concept of the translational tuning of protein folding. The focus of this meeting will be on nascent polypeptide chain structure and folding, regulation of translation, and nascent chain targeting and translocation to membranes.

 

 

Organizers:

Prof. Marina Rodnina Max Planck Institute for Multidisciplinary Sciences, Germany	Prof. John Christodoulou University College of London, UK	Prof. Gunnar von Heijne Stockholm University, Sweden
Prof. Zoya Ignatova University of Hamburg, Germany	Prof. Anton Komar Cleveland State University, USA	Prof. Susan Marqusee University of California, Berkley, USA

 

 

Support from:

• Dr. John Vitullo, CEO, Omega Laboratories, Inc

 

• Boehringer Ingelheim Stiftung

  

Abstract Book.pdf

Protein Folding on the Ribosome_V5_web_.pdf

Sun, December 15

4:00 PM → 5:00 PM Registration

5:00 PM → 5:15 PM Opening

Marina Rodnina

5:00 PM → 11:00 PM Session 1: FOLDING and ASSEMBLY

Chair: Marina Rodnina

5:15 PM → 6:00 PM Keynote: Molecular chaperone functions in protein folding in situ

Ulrich Hartl

6:00 PM → 6:30 PM Talk: Co-translational assembly of the multi-tRNA synthetase complex

Paul Fox

6:30 PM → 8:00 PM Dinner

8:00 PM → 11:00 PM Reception

Informal discussions at Harnack House

Mon, December 16

9:00 AM → 9:40 AM Focus Talk: The ribosome lowers the entropic penalty of protein folding

John Christodoulou

9:00 AM → 2:00 PM SESSION 2: INSIDE THE TUNNEL

Chair: Gunnar von Heijne

9:40 AM → 10:10 AM Talk: Flip-flop binding in the exit tunnel of the ribosome-targeting antimicrobial peptides

Alexander Mankin

10:10 AM → 10:25 AM Short Talk: Cotranslational Protein Folding Through Non-native Structural Intermediates 1

Amir Bitran

10:25 AM → 10:40 AM Short Talk: Cotranslational Protein Folding Through Non-native Structural Intermediates 2

Siyu Wang

10:40 AM → 11:10 AM Break

11:10 AM → 11:40 AM Talk: Deciphering the dynamics of divergent co-translational assembly pathways

Ayala Shiber

11:40 AM → 12:10 PM Talk: A translation ruler to probe early cotranslational folding

Marina Rodnina

12:10 PM → 12:40 PM Talk: The importance of the N-terminus is nascent chain folding

Susan Marqusee

1:00 PM → 2:00 PM Lunch

2:00 PM → 8:00 PM SESSION 3: DOMAIN FOLDING

Chair: Zoya Ignatova

2:00 PM → 2:30 PM Talk: Cotranslational folding of disulfide rich proteins – an RBD domain story

Eugene Shakhnovich

2:30 PM → 3:00 PM Talk: Early domain folding and interactions in ABC transporter CFTR

Ineke Braakman

3:00 PM → 3:30 PM Talk: Mapping Protein-Protein Interactions at Birth

Silvia Cavagnero

3:30 PM → 4:00 PM Break

4:00 PM → 4:15 PM Short Talk: Coordination of molecular chaperones during cotranslational folding of a multidomain protein

Alzbeta Roeselova

4:15 PM → 4:30 PM Short Talk: Following the co-translational folding of a multidomain protein

Ane Metola

4:30 PM → 5:00 PM Talk: When protein folding and assembly fails: membrane protein quality control at the ER

Rebecca Voorhees

5:00 PM → 5:30 PM Short Talk: Cotranslational protein misfolding and mis-assembly on translating ribosomes

Lisa Cabrita

5:30 PM → 6:00 PM Talk: Nascent Chain-Mediated Ribosomal Frameshifting During Membrane Protein Translation

Jonathan Schlebach

6:30 PM → 8:00 PM Dinner

8:00 PM → 11:00 PM Poster Session 1

Tue, December 17

9:00 AM → 11:00 AM SESSION 4: EMERGING METHODS

Chair: Susan Marqusee

9:00 AM → 9:30 AM Talk: Visualisation of protein biogenesis in mammalian cells

Juliette Fedry

9:30 AM → 10:00 AM Talk: Protein Folding: Queerer than we suppose, or queerer than we can suppose

Stephen Fried

10:00 AM → 10:30 AM Talk: Stabilities of ribosome-bound and soluble proteomes measured by rates of methionine oxidation

Sina Ghaemmaghami

10:30 AM → 11:00 AM Break

11:00 AM → 1:00 PM SESSION 5: NC INTERACTIONS

Chair: Susan Marqusee

11:00 AM → 11:30 AM Talk: Tracking protein co-translational folding with single mRNA resolution in living cells

Ning Zhao

11:30 AM → 12:00 PM Talk: AP Profiling resolves co-translational folding pathways and chaperone interactions in cells

Christian Kaiser

12:00 PM → 12:40 PM Focus Talk: Principle of cotranslational protein targeting to mitochondria

Shu-ou Shan

1:00 PM → 2:00 PM Lunch

2:00 PM → 11:00 PM SESSION 6: TRANSLATIONAL TUNING

Chair: Anton Komar

2:00 PM → 2:30 PM Talk: Vectorial appearance induces conformational biases that alter protein folding yield

Patricia Clark

2:30 PM → 3:00 PM Talk: Uncovering Model protein characteristics that are Codon and Codon-Pair optimized

Chava Kimchi-Sarfaty

3:00 PM → 3:30 PM Talk: Synonymous but Not Silent: The Codon Usage Code for Gene Expression and Protein Folding

Yi Liu

3:30 PM → 4:00 PM Break

4:00 PM → 4:30 PM Talk: Dwelling of ribosomes at rare arginine codons and production of chaperones connect the Puf3 pumilio family RNA binding protein and Not4 to protein homeostasis

Martine Collart

4:30 PM → 4:45 PM Short Talk: Short-circuiting the bacterial ribosome: RAPPing about hydrogens 1

Martino Morici

4:45 PM → 5:00 PM Short Talk: Short-circuiting the bacterial ribosome: RAPPing about hydrogens 2

Felix Gersteuer

5:00 PM → 5:15 PM Short Talk: Mechanisms of Translational Stalling and Recovery in Bacterial Ribosomes Studied by MD Simulations

Lars Bock

5:15 PM → 5:45 PM Talk: New functional insights into SRP-Mediated Protein Targeting to the Endoplasmic Reticulum

Guenter Kramer

5:45 PM → 6:25 PM Focus Talk: Impaired biogenesis of basic proteins impacts multiple hallmarks of the aging brain

Judith Frydman

6:30 PM → 8:00 PM Dinner

8:00 PM → 11:00 PM Poster Session 2

Wed, December 18

9:00 AM → 2:00 PM SESSION 7: TRANSLATIONAL TUNING

Chair: John Christodoulou

9:00 AM → 9:30 AM Talk: Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequences

Olivia Rissland

9:30 AM → 10:00 AM Talk: The yeast E3 ligase Hel2 monitors the duration of ribosome stalling

Sabine Rospert

10:00 AM → 10:30 AM Talk: The ribosome synchronizes folding and assembly to promote oligomeric protein biogenesis

David Balchin

10:30 AM → 11:00 AM Talk: How Amino Acid Chemistry at the Ribosome A- and P-sites Modulate Translation Elongation Speed

Ed O'Brien

11:00 AM → 11:45 AM Keynote Talk: Profiling analysis of co-translational folding and assembly of newly synthesized proteins

Bernd Bukau

11:45 AM → 12:00 PM Closing Remarks

Closing remarks and poster awards

12:00 PM → 1:00 PM Informal Discussions

12:30 PM → 1:30 PM Lunch